Mechanism of RNA export in EVs

Mechanism of RNA export in EVs

We previously initiated a project aiming to determine the impact of microRNAs secreted by the endothelium on tumor development. Depending on the tumor type and context, Evs released in the tumor microenvironement show altered content in non coding RNA. Using RNA sequencing profiling of EVs we identified several non coding RNA that are specifccaly exported during cancer.

Understanding how microRNAs and non-coding RNA are specifically exported in vesicles is currently an important question in the field that we would like to tackle in the future. Using a combination of RNA cross linking and proteomic approach we identified  RNA-binding proteins involved in the export. We will continue to explore the mechanisms leading the export of other types of RNA (LncRNA, microRNA...) that we found in exosomes.

People involved : Kubra Bekar

Selected publications:

O’Grady*, T., Njock*, M.-S., Lion, M., Bruyr, J., Mariavelle, E., Galvan, B., Boeckx, A., Struman*, I., and Dequiedt*, F. (2022). Sorting and packaging of RNA into extracellular vesicles shape intracellular transcript levels. BMC Biol. 2022 201 20, 1–21.

Pérez-Boza, J., Lion, M., and Struman, I. (2018). Exploring the RNA landscape of endothelial exosomes. RNA 24, 423–435.

Pérez-Boza, J., Boeckx, A., Lion, M., Dequiedt, F., and Struman, I. (2020). hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells. Cell. Mol. Life Sci. 77, 4413–4428.